2氨基吖啶酮厂家-2氨基嘧啶

Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus

为了进一步证实上述发现，我们表达并纯化了gst - gp130胞浆区融合蛋白和gst - tle1独特性片段融合蛋白，并制备了特异性抗tle1多克隆抗体。

On cytomembrane ; resolved the precipitation in buffer d ( contained triton x - 100 ) shattered by ultrasound , 4 vibrated for 60min , centrifuged at 45000g for 1 hour , took the supernatent to mesure protein concentration and then mesure the pkc activity

目前，在铅的神经毒性研究过程中，铅对pkc的作用引起人们极大关注。这是因为铅比钙对pkc有更高的亲和力。现已知， ph卜可以取代caz ”激活蛋白激酶c 。

The activity of pkc on cytomembrane in mice brain showed ; 1 ) the value of pkc activity was lower than that of cytopla *** a , 2 ) the activity of each group reached the peak at p22 , 3 ) the value of pkc activity varied with lead concentration , especially for the 9 . 6mmol / l group

2 ）铅暴露小鼠脑细胞膜pkc活性总体上比胞浆低， pkc活性高峰时段在p22左右，胞膜pkc活性随铅浓度的升高而升高。其中9 6nunoul组升高最为明显。

To investigate the consequence of this interaction , aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector . confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus

在构建了红色荧光蛋白aes表达载体后，将其与tle绿色荧尤蛋白载体共转染细胞，共聚焦显微镜观察发现这两种分子在胞浆中有共存现象，而且aes的表达可抑制tlei向胞核内的聚积。

[ result and discussion ] 1 . bination beeen 2 - amac labeled opgochitosan and macrophage : 2 - amac - opgochitosan first bound the cytomembrane of macrophage , and then diffused in the whole cytopla *** , at last entered the nucleolus and diffused in the whole cell . fluorescence intensity increased with time

2 -氨基吖啶酮标记的壳寡糖与巨噬细胞的结合情况： 2 -氨基吖啶浙江大学硕士学位论文酮标记的壳寡糖先与巨噬细胞膜有结合，然后分布于整个细胞质，最后进入细胞核，随时间的进展而呈现了一个内在化过程。